Materials and Methods:

     3 female Eublepharis macularius and 8 male Eublepharis macularius were used in this experiment. The 3 females and one male were studied in a 40 gallon breeder tank with paper towel roll hides, cork bark, a water dish, and a circular pink container holding eggs in the center for incubation. 3 males were kept separately in foot long plastic Tupperware with paper towel substrate and a water dish. One male was in the 40 gallon tank and the other 4 were in a 30 gallon  breeder tank together on sand and with a water dish, each with his own hide box. Two were in the 30 for the first month, then two more were introduced.  The location of the defecation station was was recorded after at least 3 of the animals grouped together used it, or after a week in the separated males. 
     After a definite location for defecation was established in the 40 gallon tank, the topography was flipped but the station itself was untouched.  The setup remained the same for this control until a station was established, afterwards, all the liner and tank objects were cleaned with dawn dish washing detergent and isopropyl alcohol and place back in the same arrangement.  When a station was distinguished, the liner and cage furniture were cleaned again and arranged with either the hide or the water dish mirroring its previous location. After 3 rearrangements of the 40 gallon tank, 3 arrangements of a large hide box location were added.
     After a week of  the foot long containers, the the defecation stations were determined and the males were relocated to circular containers with no corners but otherwise similar conditions.  Defecation stations were determined after another week had passed.
     Nothing was rearranged in the 30 gallon tank other than adding in two more males with two more hide boxes. The sand in their tank was sifted every week, but not washed.